As part of the International Liver Congress (ILC) 2021, the EASL Takeaways take a look at key topics from the congress, with leading experts discussing the most impactful science and putting it into perspective.
Professors Cecília Rodrigues, Rui Castro and Saskia van Mil highlight basic science studies that used ground-breaking techniques and tools in the study of liver diseases. The first techniques examined by the experts are shotgun metagenomic sequencing and single-cell analysis, led by Professor Rodrigues. Professor Castro then discusses a study that used RNA sequencing, before Professor van Mil explores techniques that analyze single cell transcriptomics while taking into account the spatial organisations in tissues. Professor Rodrigues then presents a segment on the single-nucleus RNA-seq2 technique, which enables deep characterization of nuclei isolated from frozen archived tissues. Finally, the experts discuss liver organoids, which are increasingly used to model aspects of liver biology in vitro.
